Journal:

Article Title: Skeletal muscle dictates the fibrinolytic state after exercise training in overweight men with characteristics of metabolic syndrome

doi: 10.1113/jphysiol.2002.036616

Figure Lengend Snippet: A, QMF RT-PCR analysis of CTAP III versus a control gene (LG1) shows strong induction of mRNA in longitudinal biopsies from an exercised male metabolic syndrome patient. B, quantification of CTAP III mRNA in longitudinal biopsies from three males shows increased CTAP III mRNA expression levels in skeletal muscle after exercise training then a rapid decrease by 2 weeks detraining. C and D, results of CTAP III protein immunoblots of longitudinal samples from two subjects normalized (to myosin). CTAP III protein expression paralleled mRNA expression, with loss of CTAP III protein within 96 h of detraining. Data are ±s.e.m.; P value was determined using Student's t test.

Article Snippet: Membranes were then incubated for 2 h at room temperature with primary antibodies (t-PA and PAI-1 antibodies (Santa Cruz Biotech); β-thromboglobulin/CTAP III antibody (Affiland, Liége, Belgium); tetranectin antibody (AntibodyShop, Copenhagen, Denmark)), washed and incubated for 1 h at room temperature with peroxidase-conjugated secondary antibodies.

Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Western Blot